Saturday 28 December 2019

Biodiversity and Habitat - How we may support biodiversity as we grow food and other resources in our regenerative landscapes.

Last season we started a new survey in an attempt to gain an understanding of the biodiversity within various habitats of a regenerative landscape. During this post I'll introduce the garden we carried out the surveys in, overview the habitat types within the garden that we looked at, I'll present the survey protocol, share the results with you from the late Summer Survey and end with some discussion of the survey and results.

The Polyculture Project Habitat Survey

 So let's start with what's the point of recording biodiversity in the first place.

Why do we need to record biodiversity in the first place?

Our project mission is to develop and promote practices that can produce food and other resources for humans while enhancing biodiversity. To encourage biodiversity in our gardens, we include a variety of habitat into the landscape design, including native wild habitats (at various stages of succession) and cultivated habitats. The gardens are, essentially, a mosaic of habitat. Seeing as we are looking to enhance biodiversity within our gardens we must have the means to record the diversity within the various habitats. Our aim is to use this information to guide our land management practices specifically in terms of what ratio of land we should dedicate to wild native succession habitats and to what degree we can work within these habitats without disturbing the suitability of the habitat for other organisms i.e without disrupting the biodiversity. Furthermore, we would like to see how our cultivated habitat types i.e annual and perennial polyculture, compare to the wild habitat.

Various habitats within our Polyculture Market garden Plot - Aponia (Aquatic habitat not included)
See here for this gardens location on our project map. For habitat, description see below 

To do this completely we would need to include plants, fungi, animals (invertebrates, birds, mammals, reptiles, amphibians) and microbes. Quite a task by any standard and currently beyond the capacity of most people and projects, including ours. Actually, we do have good general records of plant - mammal - bird - reptile and amphibian species that inhabit our gardens (Dylan has started to add these to our website that you can find here if you are interested). For seasonal surveying and tracking changes over time, I believe that a good way to assess the general biological diversity is to study Invertebrate diversity i.e spiders, insects, bees, etc. Invertebrates largely rely on plants for food and shelter and Invertebrates are often relied upon by birds and mammals further up the food chain as they make up a significant portion of the larger animal's diet. It seems that within our environment at least when you have high levels of invertebrate diversity you will likely have high levels of plant and larger animal (vertebrate) diversity too.

Just a few of the organisms that inhabit our gardens 

As a side note, I believe strongly that a global standard for accurately recording and accounting for biodiversity is probably the most important factor for transitioning into regenerative agriculture and without this standard, regenerative agriculture will most probably remain a trendy, marginal application meeting only a tiny percentage of global food demand. With a global biodiversity recording and accounting standard established it can be used across the planet to provide incentives for activities that are enhancing or at the very least sustaining biodiversity levels and withdraw incentives and issue penalties for those activities that result in lowering biodiversity. This is a huge discussion beyond the scope of this post and the development and application of such a standard is something that humans, with skills that I certainly do not possess, could develop and would involve engineering new hardware and software technologies and the coordination of a massive global academic and political collaborative effort. Certainly not beyond our collective reach but probably one of the tougher challenges of our time. 

For now, I will focus on what I can immediately do and so have aimed to develop a super simple low tech method to survey invertebrate diversity within our garden habitats. The goal of the survey is that it should be simple enough that anyone can use it and that the data collected should be good enough to draw some useful conclusions.

First, an overview of the garden where we are carrying out the Surveys.

Garden Overview

Location: Shipka, Bulgaria, Southeast Europe
​Climate: Temperate
Köppen Climate Classification - Dfc borderline Cfb
USDA Hardiness Zone: 5b (conservative) - 7a (risky)
Latitude: 42°
Elevation: 565 m
Average Annual Rainfall: 588.5 mm
Prevailing Wind: NW & NE
Garden Name: Aponia - Polyculture Market Garden
Garden Location on our Project Map - See here 

Within this garden, we have identified a number of pre-existing habitats and have created some habitats. What follows is a description of those habitat types within the garden.

Habitat Types 

Habitat types refer to distinctly different regions within an area and in this case, are determined by the composition and age of the plant species within those areas. We have identified 6  habitat types within this garden that fall under the following  categories - Wild HabitatSemi Wild Habitat, Semi Cultivated Habitat, and Cultivated Habitat.

Wild Habitat Type
Late Scrub
Early Scrub

Semi Wild Habitat Type 
Mixed Species Meadow

Semi Cultivated Habitat Type
Mixed Species Hedgerow

Cultivated Habitat Type
Perennial Polyculture
Annual Polyculture

It should be noted that the cultivated habitat types as presented above were abandoned horticultural land and were heavily grazed by tethered horses before we started the development of the garden. This land, if left unattended, would succeed to a habitat very similar to the other habitats on the site namely - Mixed Species Meadow to Early Scrub to Late Scrub and would eventually form Woodland. When working with habitat types within your landscape it's important to have a good understanding of the successional pathway of the vegetation in order to properly appreciate the dynamics of the land and the species that inhabit each successional stage. This information will help you manage the land, specifically the potential for biodiversity, properly.
An excellent classification system for habitat type has been developed The UK Habitat Classification and can be downloaded from the bottom of their homepage hereOur classification system follows this broadly but I have made some adaptions to make it more relevant to our polyculture design, implementation, and management strategy. 

The map below shows these various habitat types within our 8-year-old Market Garden, Aponia.

Habitat Type Detail

The following information only includes a sample of the diversity of plant species within each habitat for the month that we carried out the survey i.e August. We intend to make a full record of all the species in each habitat across the season (to account for the seasonal succession of annual and ephemeral species) next year during our Polyculture Study 2020 and provide labeled illustrations of the placement of plants within the cultivated habitats.






Micro habitat

Our garden designs also include a variety of microhabitats such as rock piles, small and large ponds, gravel pathways, stick piles, rotten log piles, helping mats, tree stumps. These are often placed within a variety of habitat types and are not considered in the survey. A survey of our microhabitats will make a good continuation of this study for the future. 

Micro habitat from the gardens 

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So now let's look at the Survey Design and how we aim to gain a measure of biodiversity within each habitat type.

Our Survey 

Each of the 6 habitat types are surveyed for the number of unique species observed in 2 pitfall traps within each habitat type and 10 canopy observations at different locations within each habitat type.  These surveys are carried out at the same time every first and second Monday and Tuesday of the months April to September over a period of 3 years.

The time and date - weather conditions (cloud cover - temperature - wind ) and rainfall received since the last survey are all recorded before we start. (rainfall received since the last survey is only recorded for weeks 2 and 3 of each month)

The Pitfall Traps

Two pitfall traps are set within each habitat type. Each trap is labeled for recording purposes. The traps are set on Monday morning, left overnight and emptied onto white trays on Tuesday morning.

The pitfall traps labeled ready to take a position in the habitats.

The number of unique species is counted within each trap and an average of the two traps per habitat is taken to provide the average number of unique species found within each habitat type. After emptying the traps, lids are placed on the jars and the survey is repeated the week after.  The below image shows the locations of the traps within each habitat type. 

Simon setting a pitfall trap

Shahara Khaleque made a couple of videos that show the habitat types and how we undertake the pitfall traps surveys.  You can find part 1 here and part 2 here. Thank you Shahara :) 

 Canopy Observations 

A 50 m trail within each habitat is determined as shown by the red dotted lines in the below image and 10 locations labeled 1-10 are fixed 5 m apart along the trail.

At each location the observer stands and observes within their 2 -3 m field of vision (without turning head) for 2 minutes -1 min standing - 1 min kneeling and records the number of unique species they can see on the plant vegetation and on the ground. 

Protocol Invertebrate Survey - Canopy Observations 

  • Find your starting mark
  • Set timer to 2 minutes
  • Face the habitat and count the number of different species* within your 2-3 m field of vision without turning head. 1-minute standing and 1-minute kneeling 
  • After 2 minutes walk to the next mark 
  • Repeat 2 to 4 until you have completed 10 surveys. 

Species you have seen in previous locations are counted again (start new species-count after every change of location)

*Different species = Do not count individuals, count visibly different species, e.g. :
3 black ants and 1 red ant = 2 species
1 big black spider and 1 small black spider, hard to say if it is young/old or male/female = 2 species
1 small spider and 1 big spider that are clearly the same species = 1 species

Flying species: Bypassing flying insects do not count. Insects hovering (to feed on nectar/pollen) or landing in your field of vision do count.

Here is Shahara taking canopy observations in the  Early Scrub Habitat 

To account for discrepancies between individuals' vision and interpretation of what should be counted as individual species we undertake the canopy survey twice with a different person counting a different habitat and rotate who does which habitat each week. 

The data for both surveys are recorded on printed sheets as seen below. If you would like copies of the sheets, contact us via the form at the end of the post.

How we process the data 

The data gathered for all of the surveys in each habitat is averaged. For example, in the Late scrub if five unique species were counted in pitfall trap 1 and three unique species counted in trap 2 the total record for pitfall traps in this habitat would be 4.  Likewise, if eight species were counted during canopy obs 1 and four species were counted during canopy obs 2 the total record for the canopy observations for this habitat would 6 species. 

We then take an average of both the pitfall trap and the canopy surveys within each habitat for the overall species diversity of each habitat type. 

Finally, the results from each month are averaged to give us a monthly average of the number of unique species in each habitat and the monthly results are averaged to give a season average for each habitat.

Hope that all makes sense 


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Weeks 1-3 - Results Summary - Based on the average number of unique species counted in Pitfall and Observations
Week 1 - 1 day after significant rainfall Week 2 - 8 days after rainfall Week 3 - 15 days after rainfall
Rank HabitatNumber of Species Rank HabitatNumber of Species Rank HabitatNumber of Species
1Annual Polyculture 7.5751Annual Polyculture 3.7751Annual Polyculture 3.825
2Perennial Polyculture 6.52Mixed Species
3.4252Mixed Species
3Mixed Species
5.83Early Scrub2.93Early Scrub3
4Mixed Species
4.5754Perennial Polyculture 2.5254Perennial Polyculture 2.3
5Early Scrub4.25Mixed Species
2.25Mixed Species
6Late Scrub1.856Late Scrub1.76Late Scrub1.575

The August survey revealed that the Annual Polyculture was the habitat that showed the highest levels of unique invertebrate diversity and that Late Scrub showed the lowest levels of diversity.

Total - Average for each habitat (rounded up)
Rank HabitatNumber of Species
1Annual Polyculture 5.0
2Mixed Species
3Perennial Polyculture 3.8
4Early Scrub3.4
5Mixed Species
6Late Scrub1.7

Table showing the Number of unique species recorded within each Habitat Type

It's obviously too early to draw any conclusions based on 1 month of surveying but it looks like a promising start for our cultivated habitats. I think a significant part of the reason why invertebrate diversity was higher in the cultivated areas is that they are irrigated at least bi-monthly and water is probably the single most important factor concerning biodiversity especially the ground invertebrates. I expect that the early spring surveys will tell a different story with the abundant rainfall bringing life to the Scrub areas. The mixed-species meadow results were as expected and even without irrigation were teaming with flying insects taking advantage of the pollen and nectar resources on offer.

Shortcomings of the Surveys 

Our aim is to keep the survey as simple and replicable as possible without sacrificing the validity of the data. It seems to me that the barriers to entry for the majority of people when carrying out such a survey will be costs, expertise and time. I believe we have certainly addressed the cost and expertise barriers with the design of this survey and have reduced the amount of time needed to carry out the survey to something that is quite manageable providing you have a small team of 4- 6 people that are willing to give 40 minutes or so of their time 12 times a year and you are prepared to co-ordinate the group and process the data. (I've designed the sheets to do the math and punch out the results so all you need to do is enter the data). It's also possible for one person to complete the survey alone but it's almost certainly going to be tedious. Of course, surveying six habitats is not necessary and you could carry out the survey on however many you may have. Let's have a look at the shortcomings of the survey.

Here are the main shortcomings we have identified so far. If you can see more please do let us know and better still provide a solution that fits within the aim of the survey design. 

  • Perhaps that biggest shortcoming is that we do not gather information about the species. For future surveys we will develop a simple category ID list for i.e ants - bees - winged insect - snail - slug - beetle, etc. based on the most common species in order to have a better understanding of the organisms within each habitat and hopefully to identify how these organisms relate to productive capacity i.e pests, borgs (beneficial organisms), neutrals. 
  • As we are not identifying the species and have limited knowledge of entomology we may be counting unique species when in fact they are male or female, larvae/adult or at different instar stages. I expect reliable identification apps for invertebrates will be available in the not too distant future, at least for identification to the group level which can be very helpful for our purposes.
  • Because we are not using ethanol in the pitfall traps (commonly used to kill what falls in the traps), it's possible that predators may fall in and eat prey species before we can count them.
  • The canopy observations do not account for nocturnal invertebrates
  • The canopy observations from the habitat types with tall shrubs and trees do not account for invertebrates in the higher canopy. We could use a "beating tray" observation for this but for some of the habitat such as early scrub and hedgerow, it will be difficult to set this up. 
  • It could be misleading to think that just because some of the wild native habitats have lower invertebrate diversity that it is of lower value as in some cases uniformity of habitat type, although,  may not support a wide range of invertebrate diversity could be essential for certain mammals or bird that may only inhabit the area at certain intervals of the year. More study and understanding of how different organisms relate to the habitat type is required before making decisions on altering the habitat.
  • Obviously, the biodiversity metric alone cannot be used to signify environmental health. Soil health - water quality - air quality and the productive capacity in terms of meeting human needs and the stability/resiliency of the state should also be considered
 I've really enjoyed thinking about this survey and carrying it out and hope that we have managed to at least catch a glimpse of the diversity within our garden habitats and look forward to gathering a full season of data next year and beyond. If you have any suggestions on how we can improve the survey please do let us know and if you would like to try the survey yourself, send us an email and  I'll send you the record-keeping templates and protocols. You can find the complete records of the survey along with protocols, maps and habitat types here. You can access the different sheets via the blue tabs at the top of the sheet.  

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Sunday 22 December 2019

Looking for an all round unique learning opportunity? Join us for our Polyculture Study 2020.

We believe it's both possible and probably necessary for humans to be able to provide food and other resources while enhancing biodiversity. Discovering how best to achieve this is the primary focus of our work here as we undertake various trials and research in our gardens. In 2020 we shift focus from our 5-year study of annual herb and vegetable polycultures to perennial systems such as forest gardens and agroforestry. We are undertaking a multi-year study looking at the economics of running such a garden, and how productive it can be in terms of yields, income, and biodiversity. We are delighted to be offering a unique opportunity to a team of enthusiastic people to join us for our study.

Would you like to be part of the team in 2020?

What are we doing?

​We've been carrying out trials and creating demonstration gardens at our project for the last 10 years, experimenting with various polycultures and monitoring biodiversity. You can find a summary of all our results to date here on The Polyculture Project website

This season we will be developing new perennial polyculture/forest gardens to compare these with conventional organic monocultures.

The approach we take goes way beyond organic as we aim to design biological systems that rely on the native ecology to function as opposed to external manufactured inputs, and as a result, our gardens should service not only our needs but the needs of other organisms too.

 ​​The main questions we are seeking to answer during this study are:

  • Can we provide nutritious affordable food whilst enhancing biodiversity?
  • How productive can polycultures be?
  • How much time does it take to establish and manage such gardens?
  • What yields and associated income can be expected from running such gardens?
  • What advantages can polyculture provide?
  • How economically viable are these gardens? 
  • How bio-diverse can our food producing systems be and how can we measure this?​

Why are we undertaking this study?

The demand for local, biologically cultivated food appears to be on the increase, as is a general desire to promote and preserve biodiversity.  As far as we can tell based on our experience of growing polycultures for 15 years,  small scale biologically cultivated polyculture gardens seem a practical, accessible and realistic way of providing food for humans while preserving and promoting biological diversity in the environment.  With what appears to us to be a high demand and low supply situation,  we ask why are there not more of these enterprises around?  To explore the issue in more depth we are undertaking multi-year studies looking at the economics of running such gardens/farms, and how productive they can be in terms of yields, income, and biodiversity. Our end goal is to provide economically viable models that provide dignified jobs and create nutritious affordable food while enhancing biodiversity.

Why should you take part?

​This is an excellent opportunity if you are considering starting a land-based project for your own needs or commercially, and are interested in ways to provide affordable healthy food and other resources while increasing biodiversity.

As a participant of this study: -    
  • You will gain valuable insight into what it takes to actually run food-producing systems and how you can enhance biodiversity.
  • As well as the practical skills you will develop, we'll dedicate time each week to covering the essential theory of your choice, including site design and implementation, plant propagation, polyculture design and management, botany, food processing, irrigation, marketing, and advertising.
  • Enrollment for the full six months as a core team member grants your participation in all courses and training events that take place during the program free of charge. 
  • This opportunity will give you hands-on work experience and insights into what's involved in running a land-based project such as ours.
  • You will be contributing to an area of research where little information exists, i.e. the productivity of polycultures and associated biodiversity dynamics. This study will be published online and freely available to all for future reference and you will be credited accordingly.  
  • You will be spending time in a truly unique area of the world, working as part of a dynamic team of fellow enthusiasts in an inspiring environment.

Where will you be? 

The project is based in the town of Shipka, Bulgaria on the foothills of the Central Balkan mountain range in the Rose Valley. It's an area of high biodiversity, beautiful countryside and historical sites of global, cultural and scientific significance. You will be working on various sites within and around Shipka. The market garden (Aponia) and our home garden are located on the west side of the town. The team house and new gardens where we will be trialing various perennial polycultures are located on the east side of Shipka. You can find a map with the garden locations here

You'll also be learning from our existing gardens, a 14-year-old residential property with a highly productive and well established forest garden composed of over 400 species of plants.  Our central garden is a good example of an established small scale intensive ecological design and includes examples of rainwater harvesting, integrated wildlife habitat, multiple composting methods and hosts a small plant nursery. We practice various methods of biological vegetable production including guild planting and crop rotation, and during some years keep pigs, chickens, and rabbits at the property.  

What will you be doing?

You'll be working closely as a team developing trial gardens to test popular permaculture and regenerative techniques and concepts. You'll be helping to set up and maintain a market garden, growing food mainly for yourself and the team to share, but sending any surplus to a food co-operative.  For 2020 we will be looking at developing dehydrators to process fruits and experimenting with drying fruits. We will be recording all aspects of the process including how long it takes to develop, maintain and manage, the associated costs, fertility requirements return in product weight and any estimated or actual income derived from the produce.

Specific activities include the following:- 
  • Propagating crops and other plants
  • Harvesting and processing produce 
  • Record keeping - Surveying - Soil Tests  
  • Helping with food cooperative orders 
  • Managing existing and developing new beneficial habitats
  • All aspects of bed preparation for the research trials
  • Developing and maintaining new perennial polyculture trials and gardens  
We will also be recording biodiversity levels in the garden focusing specifically on model groups of invertebrates such as bees, beetles, moths and butterflies and bugs. These records are taken monthly.

The Core Team 

We are looking for a team of volunteers who are able to commit to the study and who have a deep interest/curiosity in food production and biodiversity

Desirable skills and abilities
  • To be organized and tidy: Eg, accurate record-keeping, knowing where tools belong and returning them after the morning's activities
  • Punctuality: we offer flexibility, but things run more smoothly if time is well kept 
  • Self-motivated: Able to use own initiative ​

​Desirable attributes:
  • Dedicated: willing to commit to the project
  • Open-minded: willing to experience a different culture, meet people from other cultures
  • Used to or up to the challenge of living in a small village
  • Love of the outdoors and the creatures that inhabit it!
  • Looking to expand skills and knowledge


Singles Pricing 
€1050 - core team participation for the full 6 months (shared room)
€1350 - core team participation for the full 6 months (private room)
​ €200 per month (shared room)
 €250 per month (private room)

Minimum Participation 
 €160 - 2 weeks (shared room)  
 €200 - 2 weeks (private room) 

Couples Pricing 
€1550 - core team participation for the full 6 months (shared private room)
€300 per month (shared private room)
Minimum Participation 
€240 - 2 weeks

Fees include rent and bills for the whole period, weekly training sessions, access to our spreadsheets and design files and software, admission to *courses, events and webinars held during your stay and as much as you can eat from the gardens. 
*access to  courses is only available for participants that join the study for 3 months or more 

Accommodation and Location

Our self-catering volunteer house is basic but comfortable in a beautiful location walking distance from the project sites. The house is spacious and has Wi-Fi internet, a shared kitchen, two bathrooms, and a productive garden.  ​

Fruits and vegetables produced from the gardens are available to you from May onward. The cost of living is relatively low here and estimates of living costs based on the experience of previous participants is between €120 - €180 per month.  

There is plenty to do around Shipka and our location is perfect for exploring. The wild coasts of the Black and Adriatic Sea are just a few hours away, extensive trails deep into the Balkan mountains start from your doorstep. Istanbul, Bucharest, and Thessaloniki are a bus ride away and there are great day trips including Koprinka Lake, Kalofer waterfalls,  Buzludja and Etara living Museum, to name but a few :) 

How to take part

The study will run from April 1st - September 30th and will give you hands-on work experience and insight into precisely what is involved in running a permaculture project and growing food naturally.  You will also have the option of further development by looking at expanding your specific interests such as botany, ecology, etc. by having access to specific resources and the option of giving talks and running practical sessions.  

We aim to teach you the skills needed to work with plants in a regenerative system. Activities will be largely determined by what needs doing at any particular time. We generally start at 09.00 and spend around 3 - 4 hrs a day in the gardens. Weekends are left unscheduled. There will be plenty of free time available for studying or following personal interests. 

We believe the valuable work you will bring to the project is a fair exchange for the educational experience you will receive. 

Once we have received your registration we will contact you and arrange a Skype meeting to talk through the process and answer any questions that may arise. Following this, if you decide you would like to take part, the fee for the duration of your stay should be paid in full to secure your place. Payment can be made via PayPal (processing fees apply) or bank transfer in £,€ or BGN.  Registration is now open -  we look forward to hearing from you!  

Here's what previous participants said!

"Being a part of the Polyculture Study was a very special period of my life.  I realized how inspiring it is to be in touch with soil and plants, and creatures inhabiting the garden I worked in, and also to be in good touch with the people I worked with. I am happy to have had this opportunity to support the Study and the wonderful family behind it, and to be supported by them in return." Anna Boncheva

"It made an impression on me so much so that I'm going back again to participate from the beginning to see the know-hows of food growing in cooperation with nature." Kata Prodanov

"It's a nice journey that gives you the chance to study in-depth in your free time"  Plamen Petkov

"Taking part in the Polyculture Study was a life-changing and inspiring time for me. I learned loads about permaculture, about Bulgaria and to reconnect with the simple things in life. On top of that Paul, Sophie and their kids are awesome people! I'd recommend this to anyone wanting to learn more about permaculture, experiencing rural life or just thinking of a change of career and lifestyle!"  Ute Villavicencio

Regenerative Landscape Design - Online Interactive Course 

Want to learn how to design, build and manage regenerative landscapes?  Join us for our Regenerative Landscape Design - Online Interactive Course from May 1st to Sep 13th, 2023. 

We're super excited about running the course and look forward to providing you with the confidence, inspiration, and opportunity to design, build and manage regenerative landscapes, gardens, and farms that produce food and other resources for humans while enhancing biodiversity.

Regenerative Landscape Design Online Course

You can find out all about the course here and right now we have a 20% discount on the full enrollment fees. Just use the promo code
 RLD2023 in the section of the registration form to receive your discount. 

We are looking forward to providing you with this unique online learning experience - as far as we know, the very first of its kind. If you are thinking of reasons why you should do this course and whether this course is suitable for you, take a look here where we lay it all out. Looking forward to it!


We offer a diversity of plants and seeds for permaculture, forest gardens and regenerative landscapes including a range of fruit and nut cultivars. We Deliver all over Europe from Nov - March. - Give a happy plant a happy home :)

Our Bio-Nursery - Permaculture/Polyculture/ Regenerative Landscape Plants 


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